A total of 88 selective primer combinations were screened using bulked males and females sampled from four families of Salix viminalis.A total of more than 1000 polymorphic fragments was obtained, of which only four cosegregated with sex.Brain development during childhood and adolescence is characterized by both progressive myelination and regressive pruning processes.However, sex differences in brain maturation remain poorly understood.So I would ping him occasionally, just enough to pique his interest and dangle the carrot of a possible relationship without ever actually following through with plans.

With the advent of the molecular age, the situation is changing rapidly but we are still far from a global picture of plant sex determination mechanisms and their evolution (Grant et al, 1994; see Ainsworth, 1999,2000).

He’d suggest dates, but plans would magically fall through.

and I had gone on maybe three dates, but we were still exchanging the occasional text months after the last time we saw each other. Instead, we were engaged in this bizarre textual limbo.

These four sex-linked markers were subsequently scored in individuals that were used for bulked sample preparation in additional individuals of the same families, and in individuals in other families.

A pair of primers that amplified the sex-linked fragments was constructed from one of the sex-linked amplified fragment length polymorphism (AFLP) fragments.